Colloidal gold probe-based immunochromatographic assay for the rapid detection of lead ions in water samples

Tang, Yong; Zhai, Yi-Fan; Xiang, Jun-Jian; Wang, Hong; Liu, Bin; Guo, Chang-Wei

Colloidal gold probe-based immunochromatographic assay for the rapid detection of lead ions in water samples

2010

One-step immunochromatographic assay (ICA) has been developed using colloidal gold-labeled monoclonal antibody probe for the rapid detection of lead ions in water samples. The ICA was based on the theory of competitive reactivity, and the results can be easily judged based on the presence or absence of a red colored test line with visual detection. Under optimal conditions, this method shows high detecting sensitivity with a LOD (limit of detection) of 50 ng/ml. Stability test indicates that the immunochromatographic strips are stable for 8 weeks at room temperature. During practical application, nanometer TiO2 is used to enrich the lead ions in water samples. The ICA is successfully applied in the measurement of lead ion concentrations in local water samples, and the results are highly consistent with that of ICP-MS. Detecting lead ions with ICA can be done within 4 min and is very useful for the rapid onsite testing.

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Mots clés AGROVOC

analysis antibodies antigens chemistry chromatography environmental monitoring gold immunoassay ions ions lead lead mass spectrometry methods microscopy monoclonal antibodies temperature titanium dioxide

Informations bibliographiques

Publié dans

Environmental pollution

Volume 158 Numéro 6 Pagination 2074 - 2077 ISSN 0269-7491

Editeur

Elsevier Science Ltd

D'autres materias

Transmission; Electron; Detection limit; Ovalbumin; Instrumentation; Liquid; Water pollutants; Chemical; Monoclonal; Immunoaffinity chromatography; Gold colloid; Pentetic acid

Langue

anglais

Note

In the special section: advances of air pollution science: from forest decline to multiple-stress effects on forest ecosystem services / edited by marcus schaub, rainer matyssek and gerhard wieser.

Paper presented at the international conference on air polllution and climate change at contrasting altitude and latitude, held september 7-12, 2009, murten, switzerland.

Includes references

Type

Text; Journal Article

Sur AGRIS depuis: 2024-02-28

Format: MODS

Fournisseur de données

Cette notice bibliographique a été fournie par National Agricultural Library

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Liens

DOI http://dx.doi.org/10.1016/j.envpol.2010.03.001

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