An improved method for analysis of cholecalciferol-treated baits
1999
Mauldin, R.E. | Johnston, J.J. | Riekena, C.A.
A liquid extraction method is described that permits rapid determination of cholecalciferol (D(3)) in rodenticidal grain baits. Purified D(3) was incubated for various time periods to produce pre-D(3). Response ratios (concentration/detector response) for various concentrations of pre-D(3) and D(3) in solutions permitted generation of a correction factor for direct quantitation of pre-D(3) in solutions with a pure D(3) standard. The method has equal precision and accuracy, yet is simpler and less time consuming and requires less solvents than widely accepted methods for extracting D(3) from grain baits. Recoveries from control oat baits fortified at 0.05 and 0.75 wt % were 100.9 and 98%, respectively. A standard curve for concentrations ranging from 6.4 to 204 microgram/mL had an r(2) of 0.9999 and an intercept of zero and was linear and proportional. The method limit of detection was 2.0 x 10(-4) wt % D(3).
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