Over-expression of the Arabidopsis Na⁺/H⁺ antiporter gene in Populus deltoides CL × P. euramericana CL “NL895” enhances its salt tolerance
2011
Qiao, Guirong | Zhuo, Renying | Liu, Mingying | Jiang, Jing | Li, Haiying | Qiu, Wenmin | Pan, Luanyin | Lin, Sheng | Zhang, Xiaoguo | Sun, Zongxiu
Soil salinity is a serious problem worldwide. It is necessary to improve the salt tolerance of plants to avoid the progressive deterioration of saline soil. We showed that the over-expression of AtNHX1 improves salt tolerance in a transgenic poplar (Populus deltoides CL × P. euramericana CL “NL895”) under mannose selection. Four transgenic poplar plants were obtained. Southern blot analysis showed that the pmi gene had integrated into the genome of the poplar. RT-PCR confirmed that AtNHX1 could be expressed normally in the transgenic plants. When tested for salt tolerance by NaCl stress, we measured a 100% increase in Na⁺ content in the three transgenic lines (T18, T50, T98) significantly higher than the 33% increase seen in wild-type plants. The chlorophyll content of the transgenic plants was not altered significantly, while the chlorophyll content in the control plants showed a small decrease. MDA content was decreased in the transgenic plants. These results show that the AtNHX1 gene may enhance salt tolerance due to increased vacuolar compartmentalization of sodium ions.
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