DNA-modified graphene quantum dots as a sensing platform for detection of Hg²⁺ in living cells
2015
Zhao, Xin | Gao, Jinsuo | He, Xin | Cong, Longchao | Zhao, Huimin | Li, Xiaoyu | Tan, Feng
Detection of metal ions in living cells is significant in environmental monitoring and health risk assessment. This paper reports a simple and facile method for the detection of Hg²⁺ ions in Hela cells through fluorescence imaging, which was based on the fluorescence quenching in thymine-rich DNA modified graphene quantum dots (DNA-GQDs) in the presence of Hg²⁺. The decrease in the fluorescence intensity was attributed to the electron transfer of DNA-GQDs due to Hg²⁺ bound to the thymine bases of the DNA, resulting in a T–T mismatch hairpin structure. The method shows high selectivity and sensitivity. This present sensing platform will have broad applications in biological imaging and environmental monitoring.
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