Protein synthesis in developing ovaries of mealworm under in vivo and in vitro conditions: effects of diflubenzuron
1996
Soltani, N. | Soltani-Mazouni, N. | Quennedey, B. | Delachambre, J.
The effect of diflubenzuron (DFB) on protein synthesis in the ovaries of Tenebrio molitor was evaluated during oocyte maturation using in vitro and in vitro assays. When incorporated into the diet (5 and 10 mg g-1), DFB was found to affect both the weight, the protein levels and the incorporation of tritiated leucine into proteins of ovaries. In addition, separation of ovarian proteins by sodium dodecyl sulfate-polyacrylamide slab gels (SDS-PAGE) showed that DFB applied in vitro did not have a significant effect on the number of protein bands. When added to the culture medium (5 and 10 micrograms ml-1), DFB resulted in a slight but significant decrease in the rate of incorporation of tritiated leucine into proteins of ovaries cultured for 5 d compared to controls. These in vitro effects of DFB on protein synthesis were less marked than those observed in orally treated females.
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