Effect of bamboo shoot dietary fiber on gel quality, thermal stability and secondary structure changes of pork salt-soluble proteins | Efecto de la fibra dietética del brote de bambú sobre la calidad del gel, la estabilidad térmica y los cambios de estructura secundaria de las proteínas de cerdo solubles en sal
2019
Li, Ke | Liu, Jun-Ya | Bai, Yan-Hong | Zhao, Ying-Ying | Zhang, Yan-Yan | Li, Jun-Guang | Zhang, Hua | Zhao, Diao-Bo
The effect of bamboo shoot dietary fibre (BSDF) (0%-4%) on the gel quality, protein thermal stability and secondary structure of pork salt-soluble proteins (SSP) was investigated. The water holding capacity (WHC), breaking force and particle size were increased with the addition of 2% BSDF. Differential scanning calorimetry (DSC) analysis indicated that the addition BSDF changed the thermal denaturation temperature of myosin tail and actin, and led to the disappearance of myosin head as the increase of BSDF up to 4%. SDS-PAGE showed that the band intensity of actin was obviously enhanced by the addition of BSDF. Fourier transform infrared (FTIR) spectroscopy showed that BSDF could affect the protein secondary structure. The α-helix content was significantly decreased and the β-sheet content was significantly increased (P<0.05). In conclusion, BSDF could improve gel quality of salt soluble proteins and has a potential to be applied in meat products.
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