Bacteriological examination of specified-pathogen-free animals: a procedure for small rodents
1980
Erfle, V. | Neumann, Gudrun
Organs of 10 mice or 5 rats were pooled and homogenized. A portion of the homogenate was cultured on several selective and enrichment media. The method was tested by adding various pathogenic organisms to organ homogenates. 10³-10⁴ microorganisms in homogenates of 10 organs were readily detected. This procedure allows even a small microbiological laboratory to detect a low frequency of infected animals in a colony.
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