Evaluation of six commercial DNA extraction kits for recovery of Burkholderia pseudomallei DNA
2012
de Mello Marques, Maria Angela | Zimmermann, Pia | Messelhäusser, U. (Ute) | Sing, Andreas
Six commercially available DNA extraction kits, as well as thermal lysis and proteinase K DNA extraction were evaluated regarding bacterial inactivation, DNA yield and purity, and their use in a Burkholderia pseudomallei real-time PCR. While all methods successfully inactivated the bacteria, by measuring DNA purity and the level of detection by real-time PCR, the proteinase K method was the most sensitive.
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