Efficient short extraction and purification procedures of kinsenoside from Anoectochilus roxburghii with deep eutectic solvent by column chromatographic extraction
2022
Yuan, Xin | He, Ni | Hou, Yi | Lai, Min-Ting | Hu, Song-Qing
Kinsenoside is the main bioactive compound isolated from the traditional Chinese herbal medicine Anoectochilus roxburghii with multiple health benefits. In this work, short extraction and purification procedures of kinsenoside from Anoectochilus roxburghii by deep eutectic solvent (DES) were established to extract kinsenoside, which is much more efficient, energy-saving and environment-friendly than conventional methods. To assess the extraction efficiency of kinsenoside by different methods, the total kinsenoside content in dried whole plants of Anoectochilus roxburghii was determined firstly as 157.77 ± 2.87 mg/g. The extraction efficiency of 98.06 ± 1.36% was obtained at 25 °C using column chromatographic extraction (CCE) with the ratio of column height to diameter (H/D) of 10 and the elution flow rate of 2 minimum volume per hour (MV/h), and 60% DES consisting of choline chloride (ChCl) and malic acid with the molar ratio of 1∶1 was used as extract solvent. Then the crude extract could be directly purified to 98% purity and ＞ 75% total recovery rate by silica gel column chromatography and semi-preparative liquid chromatography without extra separation and concentration. To our best knowledge, this is the first time that kinsenoside has been extracted from Anoectochilus roxburghii by CCE with DES. The results explore a new strategy for the extraction of natural bioactive compounds from plant materials.
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