Comparison of the BAX System PCR Method to Brazil's Official Method for the Detection of Salmonella in Food, Water, and Environmental Samples
2008
Tomazelli, Ingrid Boesche | Freitas, Josinete Barros de | Fabbi, Leania Maria | Filipini, Terezinha Agnese | Silva, Claudia Maria da | Bedin, Janaina Mussnich | Duarte, Dalila Angelica Moliterno | Santos, Amaury dos | Baccarin, Aldo | Higa, Ligia Rossi Garcia | Yano, Dirce Mithico Yamaoka | Killner, Mario | Frezza, Andrea Leao Cameiro | Abecia, Eduardo Carlos de Gosztonyi | Tronco, Vania Maria | Tomazelli Junior, Osmar
A two-stage study compared the BAX system PCR method with the reference culture method used by the Brazilian Ministry of Agriculture and Food Supply for the detection of Salmonella in food, water, and environmental samples. In stage 1, fish matrix samples (n = 258) were spiked at several levels with Salmonella and a combination of Salmonella and non-Salmonella competitive organisms. Replicates were analyzed by the BAX system PCR method and the reference method with comparable results (sensitivity >or=97.5%, specificity >or=83.3%) from both methods at the limit of detection. In stage 2, a total of 1,988 samples with 70 product types were analyzed with both methods. Five laboratories were involved in this study, and the samples used were from routine analyses. The BAX system PCR method was shown to be comparable to the reference method, with a limit of detection of 1.0 to 2.0 CFU/25 g of sample. Analysis of the results obtained in stage 2 and in the combination of stages 1 and 2 for the BAX system showed the following performance: sensitivity >or=99.0%, specificity >or=97.2%, false-negative rate <or=1.1%, and false-positive rate <or= 2.8%. Therefore, the BAX system appears to be equivalent to the reference method, with >or=97.3% agreement.
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