Duck Egg White–Derived Peptide VSEE (Val‐Ser‐Glu‐Glu) Regulates Bone and Lipid Metabolisms by Wnt/β‐Catenin Signaling Pathway and Intestinal Microbiota

Guo, Danjun; Liu, Weiwei; Zhang, Xing; Zhao, Mengge; Zhu, Biyang; Hou, Tao; He, Hui

Duck Egg White–Derived Peptide VSEE (Val‐Ser‐Glu‐Glu) Regulates Bone and Lipid Metabolisms by Wnt/β‐Catenin Signaling Pathway and Intestinal Microbiota

2019

SCOPE: Val‐Ser‐Glu‐Glu (VSEE), identified from duck egg white peptides, has been proven to facilitate calcium absorption in a previous study. Since prevention of osteoporosis is important, it might act as a potential cofactor in osteoporosis prevention. Therefore, the aim of this study is to investigate the regulation of VSEE on osteoporosis and abnormal lipid metabolisms. METHODS AND RESULTS: MC3T3‐E1 cell and ovariectomized (OVX) rat model are used to evaluate VSEE on regulation of bone and lipid metabolisms. Differentiation and matrix mineralization of preosteoblast are significantly increased by VSEE (p <0.05), which attributed to stimulating calcium influx, then to activating Wnt/β‐catenin signaling pathway and regulating runt‐related transcription factor 2 and osteoprotegerin. VSEE can cross Caco‐2/HT‐29 co‐cultured monolayer via paracellular pathway and peptide transporter 1 (PepT1), and can be detected in blood and maximum concentration is 122.84 ± 3.68 mg L⁻¹ at 60 min. Additionally, VSEE reverses bone loss and regulate dyslipidemia through Wnt/β‐catenin signaling pathway in OVX rats. Firmicutes phylum, Veillonellaceae, Prevotellaceae and six genera in VSEE group are significantly different compared with the Model group (p < 0.05). CONCLUSION: VSEE promotes bone growth and inhibit abnormal lipid metabolism in an OVX model through the regulation of intestinal microbiota compositions and Wnt/β‐catenin signal pathway.

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