Disruption of specific flavonoid genes enhances the accumulation of flavonoid enzymes and end-products in Arabidopsis seedlings

Pelletier, M.K.; Burbulis, I.E.; Winkel-Shirley, B.

Disruption of specific flavonoid genes enhances the accumulation of flavonoid enzymes and end-products in Arabidopsis seedlings

1999

Pelletier, M.K. | Burbulis, I.E. | Winkel-Shirley, B.

Polyclonal antibodies were developed against the flavonoid biosynthetic enzymes, CHS CHI, F3H FLS, and LDOX from Arabidopsis thaliana. These antibodies were used to perform the first detailed analysis of coordinate expression of flavonoid metabolism at the protein level. The pattern of flavonoid enzyme expression over the course of seedling development was consistent with previous studies indicating that chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), and flavonol synthase (FLS) are encoded by early' genes while leucoanthocyanidin dioxygenase (LDOX) is encoded by a 'late' gene. This sequential expression may underlie the variations in flavonoid end-products produced during this developmental stage, as determined by HPLC analysis; which includes a shift in the ratio of the flavonols, quercetin and kaempferol. Moreover, immunoblot and HPLC analyses revealed that several transparent testa lines blocked at intermediate steps of the flavonoid pathway actually accumulated higher levels of specific flavonoid enzymes and end-products. These results suggest that specific intermediates may act as inducers of flavonoid metabolism.

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Mots clés AGROVOC

acyltransferases arabidopsis arabidopsis thaliana biosynthesis enzymology escherichia coli flavonoids flavonoids gene expression gene expression regulation genes genetics glycosides immunoblotting immunology intramolecular lyases light metabolism mutagenesis mutants mutation oxidoreductases plant plant quercetin recombinant proteins seedlings transferases

Informations bibliographiques

Publié dans

Plant molecular biology

Volume 40 Numéro 1 Pagination 45 - 54 ISSN 0167-4412

D'autres materias

Naringenin-chalcone synthase; Base sequence; Time factors; Oxygenases; Analogs & derivatives; Isolation & purification; Amino acid sequence; Leucoanthocyanidin dioxygenase; Growth & development; Chalcone isomerase; Plant proteins; Flavonol synthase; Flavanone 3-hydroxylase; Oxygenases; Molecular sequence data; Mixed function oxygenases; Chemical constituents of plants; Kaempferols; Seedling growth

Langue

anglais

Note

2019-12-05

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-28

Format: MODS

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