Interaction of Environmental Moisture with Powdered Green Tea Formulations: Effect on Catechin Chemical Stability [Erratum: 2008 Aug. 27, v. 56, no. 16, p. 7586.]
2008
Ortiz, J. | Ferruzzi, M.G. | Taylor, L.S. | Mauer, L.J.
Green tea and tea catechins must be stable in finished products to deliver health benefits; however, they may be adversely affected by tea processing/storage conditions and the presence of other components. The objective of this study was to determine the effects of storage relative humidity (RH) and addition of other ingredients on catechin stability in simulated dry beverage mixtures. Samples of green tea powder alone and mixed with sucrose, citric acid, and/or ascorbic acid were prepared and stored in desiccators at 22 °C and 0-85% RH for up to 3 months. Epicatechin, epigallocatechin, epicatechin gallate, and epigallocatechin gallate were determined by high-performance liquid chromatography (HPLC). Formulation and the interaction of formulation and RH significantly promoted catechin degradation (P < 0.0001). The chemical degradation of total and individual catechins in green tea powder formulations was significantly increased (P < 0.0001) by exposure to increasing RH, and the degradation was exacerbated at >=58% RH by the presence of powdered citric acid and at >=75% RH by the presence of ascorbic acid. Catechins degraded the most in formulations containing both acids. Although catechin chemical stability was maintained at <=43% RH in all samples stored at 22 °C for 3 months, caking was observed in samples at these realtive humidities. These results are the first to demonstrate that addition of other dry components to tea powders may affect catechin stability in finished dry blends and highlight the importance of considering the complex interplay between a multicomponent system and its environment for developing stable products.
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