Detinning Factors from the Shoots of Bamboo Leleba oldhami
1987
CHIEN, CHIN‐HSIANG | SU, JONG‐CHING
Concentrated fresh juice of bamboo shoots was filtered through a Sephadex G‐75 column under aseptic and anaerobic conditions. The detinning activity in the filtrate was assayed by an oxygen monitor in the presence of tin powder. A large amount of peptides with molecular weights about 1300, some nucleotides and p‐hydroxybenzaldehyde were identified as the detinning substances. Twenty peptides were isolated by means of cation and anion exchange chromatography. The primary structures of these peptides were elucidated by the subtractive Edman degradation, in which a high performance liquid chromatograph was used for amino acid and sugar analysis.
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