Description of Hydrogenophaga laconesensis sp. nov. isolated from tube well water
2016
Mantri, Soniya | Chinthalagiri, Mohan Rao | Gundlapally, Sathyanarayana Reddy
A light cream color colony was isolated, using oligotrophic LB agar medium, from a water sample collected from a tube well and designated as HWB-10ᵀ. Cells of strain HWB-10ᵀ were Gram-negative, motile rods, non-spore forming, positive for catalase, oxidase, nitrate reduction and esculin. The predominant fatty acids were C₁₆:₀, summed feature 4 (C₁₆:₁ ω7c/iso-C₁₅:₀ 2-OH) and summed feature 7 (C₁₈:₁ ω7c, C₁₈:₁ ω9t and/or C₁₈:₁ ω12t), and the major ubiquinone was Q-8. NCBI-BLAST- and EzTaxon-based 16S rRNA gene sequence similarity search identified strain HWB-10ᵀ as a member of the genus Hydrogenophaga and H. atypica DSM 15342ᵀ, H. defulvi DSM 15341ᵀ, H. palleronii LMG2366ᵀ and H. taeniospiralis LMG7170ᵀ being the nearest phylogenetic species with a similarity (%) of 99.3, 99.1, 98.4 and 98.2, respectively, while the similarity was <98.0 % with other species of the genus. However, DNA–DNA similarities between HWB-10ᵀ and H. atypica DSM 15342ᵀ and H. defulvi DSM 15341ᵀ were 37.0 and 43.0 %, respectively, indicating that strain HWB-10ᵀ is a novel species. Further, the DNA fingerprinting, based on BOX-, ERIC-, (GTG)₅- and REP-PCR amplifications, distinguished strain HWB-10ᵀ from its closest species, H. atypica DSM 15342ᵀ and H. defulvi DSM 15341ᵀ with similarity coefficients of 0.45 and 0.37, respectively, a value sufficient to establish the species status within the genus Hydrogenophaga. In addition, strain HWB-10ᵀ exhibited several phenotypic differences with its closely related species. Based on the above cumulative differentiating characteristics, strain HWB-10ᵀ was identified as a new species of the genus Hydrogenophaga and proposed as Hydrogenophaga laconesensis sp. nov. with strain HWB-10ᵀ (KTCC 42478ᵀ = LMG 28681ᵀ) as its type strain.
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