Separation of cis and trans unsaturated fatty acid methyl esters by silver ion high-performance liquid chromatography
1994
Adlof, R.O.
A variety of isomeric fatty acid methyl esters were separated on a commercially available silver ion chromatography column utilizing a versatile, isocratic solvent system composed of acetonitrile in hexane and utilizing UV detection. Samples containing all possible cis and trans isomers derived from methyl oleate (cis-9-octadecenoate; 18:1), methyl linoleate (cis-9, cis- 12-octadecadienoate; 18:2), methyl linolenate (cis-9, cis-12, cis- 15-octadecatrienoate; 18:3) and methyl arachidonate (cis-5, cis- 8, cis-11, cis-14-eicosatetraenoate; 20:4) were analyzed. The cis and trans isomers from methyl oleate, methyl linoleate and methyl linolenate were resolved, as were 15 of the 16 isomers from methyl arachidonate. The separation of the 20:4 isomers exceeded the capability of capillary gas and liquid chromatographic methodologies.
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