Synthesis of substituted terpyridine ligands for use in protein purification

Lin, Chih-Pei; Florio, Pas; Campi, Eva M.; Zhang, Chunfang; Fredericks, Dale P.; Saito, Kei; Jackson, W Roy; Hearn, Milton T.W.

Synthesis of substituted terpyridine ligands for use in protein purification

2014

A number of 4,4″-disubstituted terpyridines bearing a 4′-thioethanamine linker, and regioisomers thereof, have been synthesised from 4-substituted picolinates. The substituted terpyridines were immobilised onto epoxy-activated Sepharose™ FF gel, creating functionalised solid supports for the fractionation of proteins—including antibodies—by mixed mode affinity chromatography. The metal chelating properties of the immobilised ligand render the stationary phase also amenable for use in immobilised metal-ion affinity chromatography (IMAC), and have been demonstrated with the purification of suitably tagged green fluorescent protein (GFP).

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Mots clés AGROVOC

chemical reactions chemical structure fractionation gels ligands organic compounds

Informations bibliographiques

Publié dans

Tetrahedron

Volume 70 Pagination 8520 - 8531 ISSN 0040-4020

Editeur

Elsevier Ltd

D'autres materias

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Langue

anglais

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-28

Format: MODS

Fournisseur de données

Cette notice bibliographique a été fournie par National Agricultural Library

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Liens

DOI DOI http://dx.doi.org/10.1016/j.tet.2014.09.074

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