Cyanate is transported by the nitrate permease in Azotobacter chroococcum
1996
Munoz-Centeno, M.C. | Paneque, A. | Cejudo, F.J.
Azotobacter chroococcum cells exhibiting the capacity to take up nitrate actively could transport [14C]cyanate. This activity was dependent on the nitrogen source present in the culture medium, ammonium acting as a repressor and nitrate as an inducer. The uptake of cyanate required metabolic energy and was absent from A. chroococcum TR1, a mutant strain lacking the nitrate transport system, but was present at wild-type levels in A. chroococcum E4, a mutant strain deficient in nitrate reductase. These results show that cyanate is transported by the nitrate permease in A. chroococcum and therefore [14C]cyanate may be useful as a nitrate analogue for studies on nitrate transport.
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