New perspectives on the modification of silica aerogel particles with ionic liquid used in lipase immobilization with platform in ethyl esters production
2018
Lisboa, M.C. | Rodrigues, C.A. | Barbosa, A.S. | Mattedi, S. | Freitas, L.S. | Mendes, A.A. | Dariva, C. | Franceschi, E. | Lima, ÁS. | Soares, C.M.F.
The increased demand for biotechnological processes in industrial applications requires the development of highly active and stable heterogeneous biocatalysts. For this reason, the present work aimed to study the immobilization process by physical adsorption of lipase from Burkholderia cepacia (BCL) on aerogel modified with protic ionic liquid (2-hydroxy-N-methylethanaminium pentanoate – C5). The adsorption study of BCL on aerogel modified with protic ionic liquid (Aerogel-IL) has been performed and compared with unmodified support (Aerogel-Control). It was observed that the modification of aerogel allowed a greater retention of BCL (337 mg.gsupport–1 or 977.7 U.gsupport–1) than the unmodified support (297 mg.gsupport–1 or 863.5 U.gsupport–1). Experimental data obtained in physical adsorption of BCL on Aerogel-IL and Aerogel-Control were well-fitted to Sips and Redlich-Peterson isotherm models, respectively. BCL adsorption on both supports was found to be a spontaneous process. Immobilized BCL on Aerogel-IL has shown a better thermal stability than free and immobilized BCL on unmodified support being active for more than 14 days. These results reveal that the modified aerogel prepared in this work can be a promising support to prepare stable and active biocatalysts.
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