Raman spectroscopy measurements of glucose and xylose in hydrolysate: Role of corn stover pretreatment and enzyme composition
2011
Shih, Chien-Ju | Lupoi, Jason S. | Smith, Emily A.
The effect of corn stover pretreatment on glucose quantitation in hydrolysate using Raman spectroscopy is evaluated. Dilute sulfuric-acid pretreatment results in a 20mgmL⁻¹ glucose limit of detection in hydrolysate. Soaking in aqueous ammonia pretreatment produces a 4mgmL⁻¹ limit of detection. Water, ethanol or hexane extraction of corn stover reduces the spectral background that limits glucose detection in dilute acid hydrolysate. Additionally, a Raman spectroscopy multi-peak fitting method is presented to simultaneously measure glucose and xylose concentration in hydrolysate. This method yields a 6.1% average relative standard error at total saccharide concentrations above 45mgmL⁻¹. When only cellulase is present, glucose and xylose yield were measured by Raman spectroscopy to be 32±4 and 7.0±0.8mgmL⁻¹, respectively. When both cellulase and hemicellulase were present, xylose yield increased to 18.0±0.5mgmL⁻¹. Enzymatic or colorimetric assays confirmed the validity of the Raman spectroscopy results.
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