New Isoflavonoid Glycosides and Related Constituents from Astragali Radix (Astragalus membranaceus) and Their Inhibitory Activity on Nitric Oxide Production
2011
Zhang, Li-Jie | Liu, Hui-Kang | Hsiao, Ping-Chun | Kuo, Li-Ming Yang | Lee, I-Jung | Wu, Tian-Shung | Chiou, Wen-Fei | Kuo, Yao-Haur
Twenty-four secondary metabolites, including 16 isoflavonoids, 7 astragalasides, and 1 benzoquinone, have been isolated from the roots of Astragalus membranaceus (Astragali radix). Among these isolated isoflavonoids, (−)-methylinissolin 3-O-β-d-(6′-acetyl)-glucoside (1), (−)-methylinissolin 3-O-β-d-{6′-[(E)-but-2-enoyl]}-glucoside (2), and calycosin 7-O-β-d-(6′′-acetyl)-glucoside (3) have been identified as new compounds on the basis of spectroscopic analysis; (−)-methylinissolin 3-O-β-d-glucoside (4) was isolated from the natural products for the first time. The nitric oxide (NO) production inhibitory activity of the major compounds has been assessed in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. To identify A. membranaceus, a fingerprint method was developed by using a high-performance liquid chromatography−evaporative light scattering detector (HPLC-ELSD) method. Furthermore, characteristic peaks for the 11 major compounds in the chromatogram were unambiguously confirmed.
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