Hydrogen sulfide negatively regulates cd-induced cell death in cucumber (Cucumis sativus L) root tip cells
2020
Luo, Shilei | Tang, Zhongji | Yu, Jihua | Liao, Weibiao | Xie, Jianming | Lv, Jian | Feng, Zhi | Dawuda, Mohammed Mujitaba
BACKGROUND: Hydrogen sulfide (H₂S) is a gas signal molecule involved in regulating plants tolerance to heavy metals stress. In this study, we investigated the role of H₂S in cadmium-(Cd-) induced cell death of root tips of cucumber seedlings. RESULTS: The results showed that the application of 200 μM Cd caused cell death, increased the content of reactive oxygen species (ROS), chromatin condensation, the release of Cytochrome c (Cyt c) from mitochondria and activated caspase-3-like protease. Pretreatment of seedlings with 100 μM sodium hydrogen sulfide (NaHS, a H₂S donor) effectively alleviated the growth inhibition and reduced cell death of root tips caused by Cd stress. Additionally, NaHS + Cd treatment could decrease the ROS level and enhanced antioxidant enzyme activity. Pretreatment with NaHS also inhibited the release of Cyt c from the mitochondria, the opening of the mitochondrial permeability transition pore (MPTP), and the activity of caspase-3-like protease in the root tips of cucumber seedling under Cd stress. CONCLUSION: H₂S inhibited Cd-induced cell death in cucumber root tips by reducing ROS accumulation, activating the antioxidant system, inhibiting mitochondrial Cyt c release and reducing the opening of the MPTP. The results suggest that H₂S is a negative regulator of Cd-induced cell death in the root tips of cucumber seedling.
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