Plasmid content of piliated and nonpiliated forms of Moraxella bovis

Wilt, G.R.; Wu, G.; Bird, R.C.; Toivio-Kinnucan, M.

Plasmid content of piliated and nonpiliated forms of Moraxella bovis

1990

Wilt, G.R. | Wu, G. | Bird, R.C. | Toivio-Kinnucan, M.

Plasmid profiles were compared between nonpiliated and piliated forms of Moraxella bovis isolates. The piliated form of M bovis isolate IBH64 contained 1 fewer plasmid than did the nonpiliated form. Piliated and nonpiliated cells of IBH64 contained plasmids having molecular size of 45, 32.8, 4.9, and 4.6 kilobases (kb). Single- and double-restriction endonuclease digestion by Ava I and Nde I indicated that the size of the additional plasmid carried by the nonpiliated form of IBH64 was approximately 43.6 kb. The M bovis isolates, Newport and GRS, contained the same number of plasmids in either their piliated or nonpiliated form.

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