Effect of Gel Firmness at Cutting Time, pH, and Temperature on Rennet Coagulation and Syneresis: An in situ 1H NMR Relaxation Study
2010
Hansen, Christian Lyndgaard | Rinnan, Åsmund | Engelsen, S. B (Søren Balling) | Janhøj, Thomas | Micklander, Elisabeth | Andersen, Ulf | Berg, Frans van den
The objective of this study was to monitor rennet-induced milk gel formation and mechanically induced gel syneresis in situ by low-field NMR. pH, temperature, and gel firmness at cutting time were varied in a factorial design. The new curve-fitting method Doubleslicing revealed that during coagulation two proton populations with distinct transverse relaxation times (T2,1 = 181, T2,2 = 465 ms) were present in fractions (f1 = 98.9%, f2 = 1.1%). Mechanical cutting of the gel in the NMR tube induced macrosyneresis, which led to the appearance of an additional proton population (T2,3 = 1500−2200 ms) identified as whey. On the basis of NMR quantification of whey water the syneresis rate was calculated and found to be significantly dependent on pH and temperature.
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