Improvement of butyrylcholinesterase enzyme inhibition and medicinal properties of extracts of Aristotelia serrata leaves by ultrasound extraction
2020
Majid, Hafiz | Silva, Filipa V.M.
Aristotelia serrata, wineberry, or makomako, is a small deciduous fast-growing shrub of New Zealand that produces a berry. In this study leaf extracts were produced using ultrasound technology. Alzheimer’s Disease is a progressive mental deterioration related with ageing and senility, in which approved drugs for treatment inhibit butyrylcholinesterase (BChE), an enzyme present in the human brain. The ultrasound assisted extraction of natural anti-butyrylcholinesterase and antioxidants from this plant, and the yield of extraction were optimized using response surface methodology. The effect of acoustic power density (0.13–0.65 W/mL), time (1–20 min), and ethanol concentration (20–80%) on BChE inhibition, antioxidant activity, and extraction yield was determined, the optimum extraction conditions being 0.53 W/mL, 17.12 min and 74% ethanol. The optimized extracts were produced to experimentally validate the predicted responses. The IC₅₀ concentrations were experimentally determined (26.2 μg/mL for BChE, 5.5 μg/mL for radical scavenging activity). A. serrata leaf extracts have potential to be used as complements to medicines traditionally used for memory dysfunction.
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