Enhanced Recovery and Purification of P(3HB-co-3HHx) from Recombinant Cupriavidus necator Using Alkaline Digestion Method
2012
Anis, Siti Nor Syairah | Nurhezreen, M. I. | Sudesh, K. | Amirul, A. A.
A simple, efficient and economical method for the recovery of P(3HB-co-3HHx) was developed using various chemicals and parameters. The initial content of P(3HB-co-3HHx) in bacterial cells was 50–60 wt%, whereas the monomer composition of 3HHx used in this experiments was 3–5 mol%. It was found that sodium hydroxide (NaOH) was the most effective chemical for the recovery of biodegradable polymer. High polyhydroxyalkanoate purity and recovery yield both in the range of 80–90 wt% were obtained when 10–30 mg/ml of cells were incubated in NaOH at the concentration of 0.1 M for 60–180 min at 30 °C and polished using 20 % (v/v) of ethanol.
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