Nature of the inactivation curves of Bacillus pumilus spores heated using non-isothermal and isothermal treatments
2002
Ruiz, P. | Ocio, M.J. | Cardona, F. | Martínez Fernández, Ángel | Rodrigo, M. | Martinez, A.
Isothermal and non-isothermal heat resistance studies were carried out on Bacillus pumilus spores. The non-isothermal study revealed a non-linear behavior of the survivor curve, which was revealed as a tail after an isothermal study. Results indicated that the spores obtained by isolating cells from colonies of the tail section were more heat resistant than the original ones (D(104 degrees C) = 0.15 and 1.9 min for spores from the original population and from the tail, respectively). Application of the Weibull distribution model to analyze the tail produced good results at the 3 temperatures studied. Nevertheless, when the model was applied to curves having both concave downward and concave upward sections a poor result was obtained, with an accuracy factor greater than 1.
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