Heterologous expression and characterization of a laccase from Laccaria bicolor in Pichia pastoris

Wang, Bo; Wang, Xiuying; Tian, Yongsheng; Li, Zhenjun; Gao, Jianjie; Yan, Ying; Peng, Rihe; Yao, Quanhong

Heterologous expression and characterization of a laccase from Laccaria bicolor in Pichia pastoris

2016

Synthetic dyes are known to be highly toxic to mammalian cells and mutagenic and carcinogenic to humans and, therefore, should be detoxified and removed from industrial effluents. Different approaches for removal and detoxication are extensively sought. Biochemical methods are considered the most economical and effective method of dye decolourization. In this research, the laccase gene from Laccaria bicolor was modified and expressed in Pichia pastoris. The properties of the recombinant laccase and its ability to degrade synthetic dyes were studied. The laccase activity was optimal at pH 2.2 and 50 °C. Its Kₘ value was 0.187 mmol/L for ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)]. The laccase obtained was shown to decolorize the synthetic dyes, malachite green, crystal violet and orange G, with ABTS as a mediator. These results indicated that the laccase obtained may be used to treat industrial effluents containing artificial dyes.

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Mots clés AGROVOC

biotechnology carcinogenicity decolorization equipment genes laccase mutagens ph pichia pastoris

Informations bibliographiques

Publié dans

Biotechnology, biotechnological equipment

Volume 30 Numéro 1 Pagination 63 - 68 ISSN 1314-3530

Editeur

Taylor & Francis

D'autres materias

Synthetic dyes; Malachite green; Gentian violet; Komagataella pastoris; Laccaria bicolor; Heterologous gene expression

Langue

anglais

Note

Nal-ap-2-clean

This study was supported by the Key Project Fund of the Shanghai Municipal Committee of Agriculture [grant number zhongzi2013-8], [grant number zhongzi2014-2], [grant number gongzi2014 7-1-3]; Agriculture Science Technology Achievement Transformation Fund [grant number 143919N0300]; National Natural Science Foundation [grant number 31071486], [grant number 31200212], [grant number 31200075], [grant number 31200076]; basic research in the field of science and technology project of Science and Technology Commission of Shanghai Municipality [grant number 14JC1403602]; the Growth Plan for Young Scientists from Shanghai Municipal Committee of Agriculture and the run-up project of the Shanghai Academy of Agricultural Sciences [grant number ZP18].

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-28

Format: MODS

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Liens

DOI http://dx.doi.org/10.1080/13102818.2015.1104261

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