Effects of medium composition on hirudin production in recombinant, Saccharomyces cerevisiae
1996
Choi, C.M. | Kim, M.D. | Rhee, S.K. | Seo, J.H.
The enriched medium based on yeast nitrogen base(YNB)increased hirudin synthesis and secretion in recombinant Saccharomyces cerevisiae in batch and fed-batch cultures. Fed-batch fermentation with the defined medium yielded 342 mg hirudin/l but supplementation of yeast extract increased the final hirudin concentration to 461 mg hirudin/l. The defined medium, however, produced the product protein with higher purity of 21% and hence will allow easy separation of secreted hirudin from other contaminated polypeptides present in the growth medium. In a continuous culture, the defined medium yielded higher concentrations of cell mass and hirudin than the complex medium.
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