An investigation into the occurrence of the toxic amino acid gizzerosin in fish meal
1991
Wagener, W.W.D. | Koch, K.R. | Wessels, J.P.H. | Post, B.J.
A method is presented whereby the toxic amino acid gizzerosine can be determined in fish meals to a lower limit of 10 mg kg-1. Fish meal was hydrolysed with hydrochloric acid and, after concentration by freeze drying, derivatised with dansyl chloride solution without further clean-up. A fraction containing the tridansyl derivative of gizzerosine was separated and collected by HPLC and from this the didansyl derivative was separated after treatment with formic acid on the same column as previously using the same mobile phase. Seventeen fish meals, some causing severe gizzard erosion in chickens, were analysed but in none of them could gizzerosine at or above this level be found.
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