Development of a novel engineered antibody targeting human CD123
2016
Moradi-Kalbolandi, Shima | Habibi-Anbouhi, Mahdi | Golkar, Majid | Behdani, Mahdi | Rezaei, Gashin | Ghazizadeh, Leila | Abolhassani, Mohsen | Shokrgozar, Mohammad Ali
Antibody engineering involves a range of custom modifications of immunoglobulins to improve their affinity, valency, and pharmacokinetics, ensuring a better target therapy achievement. A number of therapeutic antibodies have been used for cell surface receptor blockage, interfering with the ligand binding and inhibiting receptor-driven activation of cells. Here we describe the construction and characterization of a recombinant bivalent single-chain Fv (biscFv) that targets CD123. On conversion of anti-CD123 scFv to biscFv format, the recognition of the cognate ligand is not altered. Moreover, the increased overall efficacy of the anti-CD123 biscFv in binding and inhibition of CD123/IL-3 (interleukin-3) interactions in TF-1 cells is demonstrated.
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