A Candida albicans gene encoding a DNA ligase
1996
Andaluz, Encarnación | Larriba, Germán | Calderone, Richard
A DNA ligase‐encoding gene (Ca CDC9) was cloned from Candida albicans by complementation of an ime‐1 mutation in Saccharomyces cerevisiae. In this system, IME1 function was assayed using a S. cerevisiae strain with a ime2‐promoter‐lacZ gene fusion such that following transformation with a C. albicans genomic library, the presence of positive clones was indicated upon the addition of X‐gal to sporulation media. Transforming fragments were subcloned in pGEM7 and sequenced. Sequence homology with several ATP‐dependent DNA ligases from viruses, fission yeast, human, baker yeast and bacteria was observed. The sequence has been deposited in the EMBL data bank under the Accession Number X95001.
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