Effect of extraction conditions on the yield, purity and surface properties of sugar beet pulp pectin extracts
2007
Yapo, B.M. | Robert, C. | Etienne, I. | Wathelet, B. | Paquot, M.
The extraction of pectins from sugar beet pulp was carried out in an aqueous acid medium under different conditions using a full two-state experimental design for three extraction parameters (pH, temperature and time). The yields of the extracted pectins ranged from 4.1% to 16.2%. Their contents in pectin constituents were 35.2-76.3% galacturonic acid, 6.8-32.9% neutral sugars, 2.0-4.2% methoxy groups, 0.8-3.8% acetyl groups, and 0.1-0.7% ferulic acid. Moreover, protein residues were present in all the extracts within the range of 0.9-6.8% and varied with the extraction conditions. On high performance size exclusion chromatography, the elution pattern of the acid-extracted pectins showed a wide molar mass distribution consisted of two relatively broad peaks. Their weight-average molar mass values determined by HPSEC-RALLS ranged widely from 20,200 to 90,100 g/mol. Most of the extracted pectins were surface-active, and some of them were quite able to produce and stabilize with effectiveness oil-in-water emulsions. Thus, it was inferred that yield, physico-chemical characteristics and surface properties of acid extracted pectins from sugar beet pulp were influenced by the extraction conditions.
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