Preparative separation of six antimycin A components from antimycin fermentation broth by high-speed counter-current chromatography
2010
Wang, Jiaoyan | Wen, Yaoming | Chen, Xiuming | Lin, Yuhan | Zhou, Jingming | Xie, Ying | Wang, Huaiwang | Jiang, Hong | Zheng, Wei
A method of using high-speed counter-current chromatography (HSCCC) was established for preparative isolation and purification of antimycin A components from antimycin fermentation broth. Six antimycin A components were successfully purified for the first time by HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (5:2:4:1, by volume). Total of 20mg antimycin A₄(a or b), 25mg antimycin A₃(a or b), 21mg antimycin A₈(a or b), 34mg antimycin A₂(a or b), 26mg antimycin A₁(a or b) and 34mg antimycin A₁(a or b) with the purities of 93.2, 98.6, 96.2, 94.1, 94.9 and 96.7%, respectively, determined by high-performance liquid chromatography (HPLC), were yielded from 200mg crude sample only in one HSCCC run.
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