Getting the Point—Mutations in Maize
2007
Weil, Clifford F. | Monde, Rita-Ann
Point mutations are important tools for understanding gene functions and genetic interactions, as well as for identifying neomorphs. The Maize Targeting Induced Local Lesions IN Genomes (TILLING) Project has been established to provide reverse genetics resources that can screen ethyl methonyl sulfonate (EMS)–mutagenized populations of maize (Zea mays L.) for individuals carrying point mutations in virtually any gene in the genome. In addition, a variation on TILLING, EcoTILLING, can be used on Maize Diversity Lines to gauge how much genetic diversity is present in maize germplasm at any given locus. The maize populations developed for TILLING, in the B73 and W22 inbred lines, also serve as an excellent and publicly available forward genetics resource.
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