In vivo response of the corpus luteum to progesterone treatment of gilts during early gestation
2020
Szymańska, Magdalena | Blitek, Agnieszka
Supplementation of progesterone (P₄) in pregnant gilts increases concentrations of circulating P₄ and stimulates the secretory activity of the endometrium. In this study, there was examination of the consequences of exogenous P₄ administration on luteal P₄ content and the expression of genes related to the corpus luteum (CL) function. Gilts with gonadotropin-induced estrus were administered daily injections of corn oil (n = 8) or P₄ (n = 8) on days 3 through 10 after insemination. The animals were slaughtered on day 12 of pregnancy to obtain corpora lutea for real-time polymerase chain reaction analyses of selected genes and for enzyme immunoassay of P₄. Injections with P₄ had no effect on the concentration of P₄ and the relative abundance of mRNA transcripts of cholesterol transport-related proteins, steroidogenic enzymes, and receptors for luteotropic factors in the luteal tissue. The abundance of prostaglandin (PG) endoperoxide synthase 2, PGI2 synthase, PGI2 receptor, fibroblast growth factor 2, peroxisome proliferator-activated receptor γ, and tumor necrosis factor α receptor type I transcripts increased after P₄ treatment. In contrast, the relative abundance of angiopoietin 2 mRNA decreased in response to P₄ administration. In summary, P₄ supplementation in pregnant gilts does not affect luteal steroidogenesis but modulates the abundance of factors related to vascular function. Given that the endometrium is the main target tissue for P₄, an indirect uterine-mediated effect of exogenous P₄ on CL function is likely.
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