Water-soluble carbohydrates in dried plant
1996
Kerepesi, I. | Toth, M. | Boross, L.
Within the framework of studying the fructan metabolism of wheat, several comparative investigations of different preparation and extraction methods were accomplished to find the most suitable methods for quantitative determination of primary water-soluble carbohydrate contents of plant material. Changes in sugar content of oven-dried samples were detailed. Oven-dried plant materials have significantly lower total water-soluble carbohydrate content than fresh ones. The decreases were higher in monosaccharide level than in fructan and glucan. Sucrose concentration of dried samples was similar to fresh ones. Changes in sugar components were different, so their ratio in the fresh samples were not identical with the dried ones. The efficiency of extraction of soluble carbohydrate could be increased by using boiling water as the first extracting solution instead of ethanol. The TLC analysis show that oven-dried plant samples contain significantly lower amount of fructans with low degrees of polymerization (DP). The conclusion is, that neither glucose, fructose, or oligosaccharide content, nor the DP of fructans of dried plant are similar to the fresh one.
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