Determination of camptothecins in DMSO extracts of Nothapodytes foetida by direct injection capillary electrophoresis
2008
Hsiao, Hsien-Yi | Cheng, Tzong-Jih | Yang, Ge-Ming | Huang, Yiren | Chen, Richie L.C.
A rapid capillary electrophoresis procedure was developed for determining the anti-cancer components, camptothecins, in Nothapodytes foetida. The hydrophobic compound was extracted from plant tissue (ca. 1 mL of DMSO for 100 mg of dried plant tissue) with a water-miscible organic solvent, DMSO, at elevated temperature (60°C). The extract was directly injected into the separation capillary (untreated fused silica, 34 cm in length, 75 μm i.d.) and analysed in MEKC mode (369 nm). Within 5 min of migration, camptothecins were successfully separated and quantified by adding organic modifiers to the running buffer (20% DMSO, 90 mm SDS in 10 mm borate buffer, pH 8.60). The linear dynamic range for camptothecin was from 5 to 400 μg/mL. This method was proven to be very suitable for monitoring the amount of camptothecins during the cultivation of the medicinal plant.
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