Content and distribution of Erythroxylum coca leaf alkaloids
1995
Johnson, E.L.
Fully expanded leaves of Erythroxylum coca var. coca Lam. (Erythroxylaceae) 35-70-d-old were harvested from 21-month-old plants grown under greenhouse conditions. Each harvested leaf was placed on a plexiglass silhouette according to its dimensions and divided into four primary sections (petiole, base, mid and anterior) and three subsections (lamina periphery, false mid-rib, and true mid-rib) to determine the distribution and content of hygrine, cuscohygrine, trans-cinnamoylcocaine, cis-cinnamoylcocaine, tropacocaine, tropinone, methyl ecgonine and cocaine. The leaf sub-sections and petiole were extracted and analysed for alkaloid content (%) by HPLC (cocaine alkaloid only) gas chromatography and GC/MS. Cocaine, methyl ecgonine and hygrine were highest in the lamina periphery with a content of 0.48, 0.46 and 0.32%, respectively. Trans-cinnamoylcocaine was pre-eminent of the cinnamoylcocaines and was most abundant in the petiole at a content of 0.24 %. Cis-cinnamoylcocaine, tropinone, cuscohygrine and tropacocaine were ubiquitously distributed throughout the leaf where the average contents were 0.14, 0.004, 0.16 and 0.04%, respectively.
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