Production of phytase by Aspergillus ficuum and reduction of phytic acid content in canola meal
1991
Nair, V.C. | Laflamme, J. | Duvnjak, Z.
Production of the phytase (EC 3.1.3.8) from Aspergillus ficuum in a submerged batch process was inhibited by high concentrations of glucose. The inhibition was overcome by applying a fed batch technique in the production of the enzyme. Tests carried out a different oxygen concentrations revealed that aeration had a beneficial effect on the production of the enzyme. The enzyme showed an optimum pH and temperature of 5.0 and 60 degrees C, respectively. Preincubation of the enzyme preparation at 60 degrees C resulted in relatively fast denaturation of the enzyme. Upon storage a 4 degrees C it lost only 15% of its activity in 5 weeks. Aspergillus ficuum also produced phytase when grown on canola meal by a solid state technique. The enzyme catalysed degradation of the phytic acid present in the meal and completely eliminated it, rendering the commodity more suitable for animal feed. An apparent 10% increase in protein content of the canola meal was noted as a result of the growth of the microorganism.
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