The antifungal activity of butylated hydroxyanizole and lysozyme
1999
Razavi-Rohani, S.M. | Griffiths, M.W.
The antifungal activity of butylated hydroxyanizole (BHA) and lysozyme alone and combined with ethylenediaminetetraacetic acid (EDTA), at different pH (3.5, 5.6 and 7), and different NaCl concentrations (0.5 to 20%) was screened on 12 fungi. The spiral gradient end point test was used to determine the minimum inhibitory concentration (MIC). BHA was effective against all the fungi tested in the range of < 12 to 460 microgram/mL. In the presence of equal amounts of EDTA, the inhibitory effect was amplified (inhibition occurred between < 12 to 200 microgram/mL). The inhibition was more pronounced at pH 5.6 and at higher NaCl concentrations especially when EDTA was present. Lysozyme alone was only effective against Fusarium graminearum PM162 and Aspergillus ochraceus MM184, but was inhibitory for all fungi, except Candida lipolitica 1591, in the presence of EDTA. The inhibition was greatest at pH 5.6 and higher NaCl concentrations, especially when EDTA was present. These studies show that the antifungal properties of lysozyme and BHA can be greatly influenced by environmental factors.
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