Shoot regeneration through routine method of callusing in sesame (Sesamum indicum L.)
2011
Shashidhara, N. | Ashoka, N. | Santosh, D.T. | Ravikumar, H. | Dhanalakshmi, T.N. | Lokesha, R. | Janagoudar, B.S.
Shoot regeneration continued to be a vital step in tissue culture attempts in sesame. Indeed, it is opined that the shoot induction via callus in sesame crop is a major bottleneck. Globally, a place, the best-achieved in vitro shoot regeneration is placed up to 46 per cent in Japan. It is sober that in India, the shoot regeneration to be very low (<2%). Hence, it was felt that at least the limit what has been reached by a Japanese scientist could be achieved in indigenous sesame genotypes. This could become a crucial breakthrough to further advance the genetic improvement of sesame through biotechnological ways like transgenics or cell lines that are resistant to biotic and abiotic stresses. In the present investigation we are able to succeed up to 66.66 per cent in vitro shoot regeneration was recorded in Gujarat till-II on MS supplemented with 2.2 mg/l NAA + 3.3 mg/l BAP and 20mM AgNo₃ followed by 50.00 per cent by Tamil Nadu Local on MS media supplemented with NAA @ 2.0 mg/lt + BAP @ 3.0 mg/lt + AgNo₃ @ 15 mM. The results have been discussed in the light of sesame improvement through biotechnological ways particularly in vitro cell line selection.
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