OsRALyase1, a Putative F-Box Protein Identified in Rice, Oryza sativa, with Enzyme Activity Identical to That of Wheat RALyase
2002
Itō, Yasuhiro | OZAWA, Akihiko | SAWASAKI, Tatsuya | ENDO, Yaeta | OCHI, Kozo | TOZAWA, Yuzuru
A rice gene, OsRALyase1, encoding a product similar to wheat ribosomal RNA apurinic site specific lyase (RALyase), was isolated and expressed in vitro. An open reading frame of the gene predicted a protein of 476 amino acid residues with 75% identity to RALyase and contained an F-box-like motif in its amino terminal region. The rice gene product expressed in a wheat-germ protein expression system had the same characteristics as its wheat counterpart, cleaving a specific depurinated site of the 28S rRNA sarcin-ricin domain.
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