Method for the isolation and liquid chromatographic determination of furazolidone in milk
1990
Long, A.R. | Hsieh, L.C. | Malbrough, M.S. | Short, C.R. | Barker, S.A.
A method for the isolation and liquid chromatographic determination of furazolidone in market milk is presented. Furazolidone-fortified or blank milk samples were blended with octadecylsilyl (C18) derivatized silica. The C18/milk matrix was used to prepare a column that was washed with hexane (8 mL) following which furazolidone was eluted with dichloromethane (8 mL). The eluate contained furazolidone, which was free from interferences when analyzed by high-performance liquid chromatography (HPLC) utilizing UV detection (365 nm, photodiode array). Extracted standard curves were linear (0.998 +/- 0.001, n = 5), and the average percent recovery (81.7 +/- 8.0%, n = 35) and intern (9.1 +/- 5.5%, n = 35) and intraassay (2.9%, n = 5) variabilities for the concentration range examined (7.8-500 ng/mL of milk, 20-microliter injection volume) were indicative of an acceptable methodology for the analysis of furazolidone. A minimal detectable limit of 156 pg (7.8 ng/mL, 20-microliters injection volume) on-column was obtained. The method uses small volumes of solvents, has a limited number of sample manipulations, and requires no pH adjustments or back-washing of extracts, making this method attractive when compared to classical isolation procedures for furazolidone.
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