Structural and functional analyses of two new S-RNase alleles, Sₛᵢ₅ and Sₐd₅, in apple
2010
Matsumoto, S. | Yamada, K. | Shiratake, K. | Okada, K. | Abe, K.
We determined the genomic DNA sequences of two new S-RNase alleles, Sₛᵢ₅ and Sₐd₅, in apple. Both consisted of 232 deduced amino acids, and contained five conserved domains (C1, C2, C3, RC4, and C5), and an intron specific to S-RNase genes of the Rosaceae, at the appropriate positions. We developed a PCR-RFLP method to identify these alleles, and clarified their occurrence in several Malus species, such as M. orientalis (W1-11-13; I.D. number 90104; Sₛᵢ₅), M. sieversii (W1-10-49; ID number 90100; Sₛᵢ₅), and M. sylvestris (ID number 88076; Sₐd₅) and cultivars, such as ‘Adersleber Calville’ (Sₐd₅) and ‘Cox’s Pomona’ (Sₐd₅). Cross-pollination tests confirmed that Sₛᵢ₅ and Sₐd₅ were functionally distinct in spite of their identical rosaceous hypervariable region (RHV), which appears to be critical for S-allele discrimination in the Rosaceae. We recommend use of the next available numbers, S₃₃ and S₃₄, instead of Sₛᵢ₅ and Sₐd₅, respectively, since the allele annotations are only transitory. This is the first known case in apple in which two S-RNase alleles containing an identical RHV act as different alleles.
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