Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico

Castro-Escarpulli, G.; Figueras, M.J.; Aguilera-Arreola, G.; Soler, L.; Fernandez-Rendon, E.; Aparicio, G.O.; Guarro, J.; Chacon, M.R.

Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico

2003

A total of 82 strains of presumptive Aeromonas spp. were identified biochemically and genetically (16S rDNA-RFLP). The strains were isolated from 250 samples of frozen fish (Tilapia, Oreochromis niloticus niloticus) purchased in local markets in Mexico City. In the present study, we detected the presence of several genes encoding for putative virulence factors and phenotypic activities that may play an important role in bacterial infection. In addition, we studied the antimicrobial patterns of those strains. Molecular identification demonstrated that the prevalent species in frozen fish were Aeromonas salmonicida (67.5%) and Aeromonas bestiarum (20.9%), accounting for 88.3% of the isolates, while the other strains belonged to the species Aeromonas veronii (5.2%), Aeromonas encheleia (3.9%) and Aeromonas hydrophila (2.6%). Detection by polymerase chain reaction (PCR) of genes encoding putative virulence factors common in Aeromonas, such as aerolysin/hemolysin, lipases including the glycerophospholipid-cholesterol acyltransferase (GCAT), serine protease and DNases, revealed that they were all common in these strains. Our results showed that first generation quinolones and second and third generation cephalosporins were the drugs with the best antimicrobial effect against Aeromonas spp. In Mexico, there have been few studies on Aeromonas and its putative virulence factors. The present work therefore highlights an important incidence of Aeromonas spp., with virulence potential and antimicrobial resistance, isolated from frozen fish intended for human consumption in Mexico City.

Afficher plus [+]

Mots clés AGROVOC

aeromonas aeromonas hydrophila aeromonas salmonicida analysis animals antibiotic resistance antibiotics chemotaxonomy classification detection dna drug resistance enzymology food contamination frozen fish frozen foods genes genetics humans isolation mexico microbiology oreochromis niloticus pharmacology polymorphism restriction fragment length polymorphism ribosomal dna rna tilapia virulence virulence

Informations bibliographiques

Publié dans

International journal of food microbiology

Volume 84 Numéro 1 Pagination 41 - 49 ISSN 0168-1605

D'autres materias

Food pathogens; Anti-bacterial agents; Gene overexpression; Food microbiology; Consumer product safety; Microbial sensitivity tests; Antimicrobial patterns; Aeromonas eucrenophila; Ribosomal; Molecular systematics; Tilapia (common name); Aeromonas punctata; Bacterial; Phenotype; Aeromonas veronii bv. sobria; Drug effects; Phenotype; Restriction fragment length; 16s; Ribosomal; Seafood

Langue

anglais

Note

2019-12-05

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-28

Format: MODS

Fournisseur de données

Cette notice bibliographique a été fournie par National Agricultural Library

Découvrez la collection de ce fournisseur de données dans AGRIS

Consulter Google Scholar

Si vous remarquez des informations incorrectes dans cette référence bibliographique, veuillez nous contacter à l'adresse [email protected]

FAO AGRIS - Système international des sciences et technologies agricoles

Share

Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico

2003

Mots clés AGROVOC

Informations bibliographiques