The establishment of a DNA fingerprinting database for 73 varieties of Lactuca sativa capitate L. using SSR molecular markers
2019
Zhou, Huiyan | Zhang, Penghang | Luo, Jiang | Liu, Xueying | Fan, Shuangxi | Liu, Chaojie | Han, Yingyan
Head lettuce originating near the Mediterranean coast was introduced to China in the fifth century. It is difficult to identify different varieties of head lettuce due to their close genetic relationships. Simple sequence repeats (SSR) technology is a practical method that has been widely used in variety identification, fingerprint construction and genetic diversity analysis. In this paper, 23 pairs of SSR primers were selected to identify and analyse 73 varieties of head lettuce. The results identified a total of 117 mutated alleles detected in 23 loci, with the number of each loci ranging from 2 to 11, with an average of 5.1 mutated alleles per locus. Additionally, 152 genotypes were detected in 23 loci, with an average of 6.6 genotypes per locus derived from a range of 2–13. The polymorphism information content ranged from 0.071 to 0.702, with an average of 0.499. The selected primer pairs could clearly reflect the genotypic diversity level of the 73 head lettuce varieties tested, and can be used in the detection and analysis of the head lettuce varieties. The results of our sequence analysis also showed that these 73 varieties of head lettuce could be effectively identified by a combination of 19 primer pairs even though the genetic similarity among the 73 varieties ranged from 0.647 to 0.991, with an average of 0.798. Based on a capillary electrophoresis platform, a DNA fingerprinting database of 73 head lettuce varieties was established.
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