Study of the Constitutively Active Form of the [alpha] Subunit of Rice Heterotrimeric G Proteins

Oki, Katsuyuki; Fujisawa, Yukiko; Katō, Hisaharu; Iwasaki, Yukimoto

Study of the Constitutively Active Form of the [alpha] Subunit of Rice Heterotrimeric G Proteins

2005

Oki, Katsuyuki | Fujisawa, Yukiko | Katō, Hisaharu | Iwasaki, Yukimoto

We used site-directed mutagenesis to engineer two constitutively active forms of the [alpha] subunit of a rice heterotrimeric G protein. The recombinant proteins produced from these novel cDNAs had GTP-binding activity but no GTPase activity. A chimeric gene for a constitutively active form of the [alpha] subunit was introduced into the rice mutant d1, which is defective for the [alpha]-subunit gene. All the transformants essentially showed a wild-type phenotype compared with normal cultivars, although seed sizes were substantially increased and internode lengths also showed some increase.

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Mots clés AGROVOC

amino acid sequences gene expression regulation genetics grain crops metabolism oryza oryza sativa phenotypic variation plant plant morphology plant structure rice

Informations bibliographiques

Publié dans

Plant & cell physiology

Volume 46 Numéro 2 Pagination 381 - 386 ISSN 0032-0781

D'autres materias

Recombinant fusion proteins; Molecular sequence data; Plant proteins; Gtp-binding protein alpha subunits; Nucleotide sequences; Plant breeding and genetics; Amino acid sequence; Heterotrimeric g-protein; Molecular sequence data; Plant physiology and biochemistry; G-proteins; Recombinant fusion proteins

Langue

anglais

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-28

Format: MODS

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Liens

DOI http://dx.doi.org/10.1093/pcp/pci036

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