AFLP MARKERS FOR THE STUDY OF INTROGRESSION BETWEEN WILD SUNFLOWER SPECIES (H. argophyllus AND H. debilis ssp. cucumerifolius) AND IN RELATION WITH H. annuus / MARCADORES AFLP PARA LA INVESTIGACION DEL INTERCAMBIO DE GENES ENTRE LAS ESPECIES SILVESTRES (H. argophyllus y H. debilis ssp. cucumerifolius) Y INSTRODUCCION DE GELNES EN H. annuus / MARQUEURS AFLP DANS L’ÉTUDE DES ÉCHANGES DE GÈNES ENTRE LES ESPÈCES SAUVAGES (H. argophyllus et H. debilis ssp. cucumerifolius) ET INTRODUCTION DE GÈNES DANS l’H. annuus
2014
Vischi, M. | Nonino, F. | Olivieri, A.M.
Sunflower is a plant of the American continent, but now many wild species are spread in different part of the world. Along the coast of the Inhambane Bay, Mozambique, two sunflower species, H. argophyllus and H. debilis, both of Texas origin, grow far apart. However, in two sites on sandy soil they grow together and many plants have been recognized as hybrids, sharing morphological traits typical of the two species. Some putative hybrid plants produced seed from which a new generation was raised at University of Udine.We present data coming from morphological and AFLP analyses of plants grown in the spring 2000. With morphological traits typical of the two species we have build up a classical hybrid index by adding the scores of each species. Clear evidence of introgressed plants results also by looking at the unequality of the AFLP bands present in H. debilis and H. argophyllus and shared in the introgressed material. A partial pollen grain staining confirms the presence of reduced fertility of some morphologically introgressed plants. From a breeding point of view, these wild species and the introgressed material seems to have great potential value for their specificity. The AFLP band pattern from H.annuus material appears to be quite unique and a cluster analysis shows a clear diversity of the three species. Deeper investigation is required in order to assess the extent of these data and to establish whether plants with intermediate traits can represent a new taxonomic unit.
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