Studies on the association of BF1/BF2 gene expression patterns with traits of genetic resistance to Marek's disease in chickens
2014
Jin, Yuan-Chang | Wei, Ping | Niu, Bo-Xue | Li, Meng | Yang, Qing-Li
The aim was try to clarify the association of BF 1 and BF 2 gene expression patterns with the traits of genetic resistance to Marek's disease (MD) in chickens. An MD-resistant line and a common line of Xiayan chickens were used in the experiment. The productions of mRNA of BF 1 and BF 2 and Marek's disease virus (MDV) gene, meq , were quantified by quantitative real-time PCR on different days post-infection (dpi) with virulent MDV in the peripheral blood leukocytes (PBLs). Results indicated that the MDV loads were lower in the MD-resistant line compared to the common line. The mRNA levels of BF 1 were significantly lower and levels of BF 2 higher in the MD-resistant line whereas levels of BF 1 were significantly higher and levels of BF 2 lower in the common line. The results demonstrated that the expression modes of BF 1 and BF 2 genes might be related to traits of genetic resistance to MD in the chicken.
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